Acidification of the culture medium as a strategy to control endophytic contaminations in Prunus spp. rootstocks cultured in GreenTray TIS bioreactor

Overgrowth of endophytes in some vitro cultures may disrupt the normal shoot tip growth and proliferation, being necessary to obtain endophytes-free achieve a plant micropropagation process. To remove these contaminations from culture medium, antibiotics are commonly added medium. However, its use production must be urgently reduced because current restrictions imposed by European Union. For that purpose, effect acidic low (pH 3) neutral 7) pH was tested GreenTray® TIS bioreactor as an alternative control without affecting Prunus rootstock RP-20 explants. The results demonstrated at 3 did not affect number shoots, FW, length amount chlorophyll pigments, but significantly population. identification also revealed Roseomonas mucosa, Microbacterium oxydans, Bacillus subtilis Luteibacter yeojuensis were bacterial isolates responsible those contaminations. These might suppose real breakthrough tissue field, although more research is required meet requirements for different species other endophytic microorganisms.